Mueller, Rainer and Eidt, Andreas and Hiller, Karl-Anton and Katzur, Verena and Subat, Michael and Schweikl, Helmut and Imazato, Satoshi and Ruhl, Stefan and Schmalz, Gottfried (2009) Influences of protein films on antibacterial or bacteria-repellent surface coatings in a model system using silicon wafers. BIOMATERIALS, 30 (28). pp. 4921-4929. ISSN 0142-9612,
Full text not available from this repository. (Request a copy)Abstract
Immobilization of defined chemical functionalities to biomaterial surfaces is employed to optimize them not only for tissue compatibility but also for prevention of bacterial infection. Grafting surfaces with chains of poly(ethylene glycol) (PEG) results in bacterial repellence whereas modification with cationic groups conveys them with bactericidal properties. Since biomaterials in situ will become exposed to a protein-rich environment, it is necessary to investigate the influence of prior protein adsorption on the antibacterial activity of this type of chemical surface modification. In the present study, we immobilized short-chain PEG and two pyridinium group-containing methacrylate monomers, 12-methacryloyloxy-dodecylpyridinium bromide (MDPB) and 6-methacryloyloxyhexylpyridinium chloride (MHPC), to silicon wafer model surfaces to investigate the influence of prior protein adsorption on the bactericidal activity of the surface coating towards subsequently attached bacteria. Adsorbed amounts of human serum albumin and salivary proteins were found to be two times higher on cationic compared to PEG-modified surfaces. An analogous tendency was found for attachment of Streptococcus gordonii and Streptococcus mutans to the same surfaces without prior protein exposure. However, most bacteria attached to cationic surfaces were found to be dead. Prior exposure of cationic surfaces to protein solutions drastically altered bacterial attachment dependent on the type of protein solution and bacterial species employed. Significantly, the original bactericidal activity of pyridinium-coated surfaces was found greatly reduced upon adsorption of a protein film. As a conclusion we propose that future approaches should combine the protein- and bacteria-repellent properties of PEG-coatings with the bactericidal function of charged cationic groups. (C) 2009 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROBIAL ADHESION; BIOFILM FORMATION; SOLID-SURFACES; ADSORPTION; BIOMATERIALS; BIOADHESION; MECHANISMS; MONOLAYERS; INFECTION; BRUSHES; Antibacterial activity; Cationic charges; MDPB; Silicon wafers; Saliva; Oral streptococci |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > PD Dr. Rainer Müller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Sep 2020 11:59 |
| Last Modified: | 03 Sep 2020 11:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28294 |
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