Hoewing, Timo and Dann, Marcel and Mueller, Benedikt and Helm, Michael and Scholz, Sebastian and Schneitz, Kay and Hammes, Ulrich Z. and Gietl, Christine (2018) The role of KDEL-tailed cysteine endopeptidases of Arabidopsis (AtCEP2 and AtCEP1) in root development. PLOS ONE, 13 (12): e0209407. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Plants encode a unique group of papain-type cysteine endopeptidases (CysEP) characterized by a C-terminal KDEL endoplasmic reticulum retention signal (KDEL-CysEP) and an unusually broad substrate specificity. The three Arabidopsis KDEL-CysEPs (AtCEP1, AtCEP2, and AtCEP3) are differentially expressed in vegetative and generative tissues undergoing programmed cell death (PCD). While KDEL-CysEPs have been shown to be implicated in the collapse of tissues during PCD, roles of these peptidases in processes other than PCD are unknown. Using mCherry-AtCEP2 and EGFP-AtCEP1 reporter proteins in wild type versus atcep2 or atcep1 mutant plants, we explored the participation of AtCEP in young root development. Loss of AtCEP2, but not AtCEP1 resulted in shorter primary roots due to a decrease in cell length in the lateral root (LR) cap, and impairs extension of primary root epidermis cells such as trichoblasts in the elongation zone. AtCEP2 was localized to root cap corpses adherent to epidermal cells in the rapid elongation zone. AtCEP1 and AtCEP2 are expressed in root epidermis cells that are separated for LR emergence. Loss of AtCEP1 or AtCEP2 caused delayed emergence of LR primordia. KDEL-CysEPs might be involved in developmental tissue remodeling by supporting cell wall elongation and cell separation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROGRAMMED CELL-DEATH; CASTOR BEAN ENDOSPERM; EXPRESSION ANALYSIS; PROTEASE; LOCALIZATION; RICINOSOMES; RICINUS; GENES; MATURATION; EMERGENCE; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Oct 2019 12:16 |
| Last Modified: | 02 Oct 2019 12:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13337 |
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