van der Linde, Karina and Egger, Rachel L. and Timofejeva, Ljudmilla and Walbot, Virginia (2018) Application of the pathogen Trojan horse approach in maize (Zea mays). PLANT SIGNALING & BEHAVIOR, 13 (12): e1547575. ISSN 1559-2316, 1559-2324
Full text not available from this repository. (Request a copy)Abstract
Maize, Zea mays, the second-most-widely-grown crop, yields 20 % of all consumed calories worldwide.(1) Despite its agronomic importance, research progress is limited by costly transformation. We recently described the Trojan horse method as a useful tool to study maize proteins in situ that circumvents time- and space-consuming whole plant transformation. The Trojan horse approach uses the protein-folding and secretory properties of the corn smut fungus Ustilago maydis to secrete maize proteins from fungal cells into the maize apoplast. Here, we discuss the timing and location of U. maydis during infection and the protein secretion site in relation to anther anatomy. This spatiotemporal analysis enables the study of apoplastic anther proteins in various premeiotic anther developmental stages, and could be adapted for larger screens.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | USTILAGO-MAYDIS; PROTEIN; SYSTEM; HOST; Maize; anther; development; cell fate; U. maydis; Trojan horse (TH); plant-pathogen interaction |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Oct 2019 08:10 |
| Last Modified: | 04 Oct 2019 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13382 |
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