Schaefer, Peter and Tueting, Christian and Schoenemann, Lars and Kuehn, Uwe and Treiber, Thomas and Treiber, Nora and Ihling, Christian and Graber, Anne and Keller, Walter and Meister, Gunter and Sinz, Andrea and Wahle, Elmar (2018) Reconstitution of mammalian cleavage factor II involved in 3 ' processing of mRNA precursors. RNA, 24 (12). pp. 1721-1737. ISSN 1355-8382, 1469-9001
Full text not available from this repository. (Request a copy)Abstract
Cleavage factor II (CF II) is a poorly characterized component of the multiprotein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactions, whereas hClp1 by itself is not. Pcf11 moderately stimulates the RNA 5' kinase activity of hClp1; the kinase activity is dispensable for RNA cleavage. CF II binds RNA with nanomolar affinity. Binding is mediated mostly by the two zinc fingers in the C-terminal region of hPcf11. RNA is bound without pronounced sequence-specificity, but extended G-rich sequences appear to be preferred. We discuss the possibility that CF II contributes to the recognition of cleavage/polyadenylation substrates through interaction with G-rich far-downstream sequence elements.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR POLY(A)-BINDING PROTEIN; AAUAAA POLYADENYLATION SIGNAL; GENOME-WIDE ANALYSIS; POLYMERASE-II; TRANSCRIPTION TERMINATION; POLY(A) SITE; IN-VITRO; CF IA; ALTERNATIVE POLYADENYLATION; SACCHAROMYCES-CEREVISIAE; 3 ' end formation; Clp1; Pcf11; mRNA processing; polyadenylation |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Oct 2019 13:18 |
| Last Modified: | 07 Oct 2019 13:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13471 |
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