Luetjohann, Dieter and Bjorkhem, Ingemar and Friedrichs, Silvia and Kerksiek, Anja and Geilenkeuser, Wolf-Jochen and Lovgren-Sandblom, Anita and Ansorena, Diana and Astiasaran, Iciar and Baila-Rueda, Lucia and Barriuso, Blanca and Bretillon, Lionell and Browne, Richard W. and Caccia, Claudio and Cenarro, Ana and Crick, Peter J. and Fauler, Guenter and Garcia-Llatas, Guadalupe and Griffiths, William J. and Iuliano, Luigi and Jesus Lagarda, Maria and Leoni, Valerio and Lottenberg, Ana Maria and Matysik, Silke and McDonald, Jeff and Rideout, Todd C. and Schmitz, Gerd and Nunes, Valeria Sutti and Wang, Yuqin and Zerbinati, Chiara and Diczfalusy, Ulf and Schoett, Hans-Frieder (2018) International descriptive and interventional survey for oxycholesterol determination by gas- and liquid-chromatographic methods. BIOCHIMIE, 153. pp. 26-32. ISSN 0300-9084, 1638-6183
Full text not available from this repository. (Request a copy)Abstract
Increasing numbers of laboratories develop new methods based on gas-liquid and high-performance liquid chromatography to determine serum concentrations of oxygenated cholesterol metabolites such as 7 alpha-, 24(S)-, and 27-hydroxycholesterol. We initiated a first international descriptive oxycholesterol (OCS) survey in 2013 and a second interventional survey 2014 in order to compare levels of OCS reported by different laboratories and to define possible sources of analytical errors. In 2013 a set of two lyophilized serum pools (A and B) was sent to nine laboratories in different countries for OCS measurement utilizing their own standard stock solutions. In 2014 eleven laboratories were requested to determine OCS concentrations in lyophilized pooled sera (C and D) utilizing the same provided standard stock solutions of OCS. The participating laboratories submitted results obtained after capillary gas-liquid chromatography-mass selective detection with either epicoprostanol or deuterium labelled sterols as internal standards and high-performance liquid chromatography with mass selective detection and deuterated OCS as internal standard. Each participant received a clear overview of the results in form of Youden-Plots and basic statistical evaluation in its used unit. The coefficients of variation of the concentrations obtained by all laboratories using their individual methods were 58.5-73.3% (survey 1), 56.8-60.3% (survey 2); 36.2-35.8% (survey 1), 56.6-59.8, (survey 2); 61.1-197.7% (survey 1), 47.2-74.2% (survey 2) for 24(S)-, 27-, and 7 alpha-hydroxycholesterol, respectively. We are surprised by the very great differences between the laboratories, even under conditions when the same standards were used. The values of OCS's must be evaluated in relation to the analytical technique used, the efficiency of the ample separation and the nature of the internal standard used. Quantification of the calibration solution and inappropriate internal standards could be identified as major causes for the high variance in the reported results from the different laboratories. A harmonisation of analytical standard methods is highly needed. (C) 2018 Published by Elsevier B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REVERSE CHOLESTEROL TRANSPORT; PICK TYPE-C; MS/MS METHOD; OXYSTEROLS; MODULATORS; METABOLISM; DIAGNOSIS; BRAIN; Oxysterol; Bile acid precursors; Gas-liquid chromatography; High performance-liquid chromatography; Mass spectrometry; Isotope dilution |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Dec 2019 14:34 |
| Last Modified: | 05 Dec 2019 14:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13729 |
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