Burla, Bo and Arita, Makoto and Arita, Masanori and Bendt, Anne K. and Cazenave-Gassiot, Amaury and Dennis, Edward A. and Ekroos, Kim and Han, Xianlin and Ikeda, Kazutaka and Liebisch, Gerhard and Lin, Michelle K. and Loh, Tze Ping and Meikle, Peter J. and Oresic, Matej and Quehenberger, Oswald and Shevchenko, Andrej and Torta, Federico and Wakelam, Michael J. O. and Wheelock, Craig E. and Wenk, Markus R. (2018) MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines. JOURNAL OF LIPID RESEARCH, 59 (10). pp. 2001-2017. ISSN 0022-2275, 1539-7262
Full text not available from this repository. (Request a copy)Abstract
Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; POLYUNSATURATED FATTY-ACIDS; STANDARD REFERENCE MATERIAL; LC-MS/MS METHOD; HIGH-THROUGHPUT; SHOTGUN LIPIDOMICS; CARDIOVASCULAR-DISEASE; METABOLOMICS RESEARCH; QUANTITATIVE-ANALYSIS; PLATELET ACTIVATION; clinical trials; diagnostic tools; lipids; mass spectrometry; absolute concentrations; clinical research; data sharing; National Institute of Standards and Technology Standard Reference Material 1950; quality control |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Dec 2019 09:57 |
| Last Modified: | 09 Dec 2019 09:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13741 |
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