Molle, Julia and Jakob, Leonhard and Bohlen, Johann and Raab, Mario and Tinnefeld, Philip and Grohmann, Dina (2018) Towards structural biology with super-resolution microscopy. NANOSCALE, 10 (35). pp. 16416-16424. ISSN 2040-3364, 2040-3372
Full text not available from this repository. (Request a copy)Abstract
Fluorescence resonance energy transfer (FRET) has been instrumental in determining the structure and dynamics of biomolecules but distances above 8 nanometers are not accessible. However, with the advent and rapid development of super-resolution (SR) microscopy, distances between two fluorescent dyes below 20 nanometers can be resolved, which hitherto has been inaccessible for fluorescence microscopy approaches due to the limited resolving power of an optical imaging system that is determined by the fundamental laws of light diffraction (referred to as the diffraction limit). Therefore, the question arises whether SR microscopy can ultimately close the resolution gap between FRET and the diffraction limit and whether SR microscopy can be employed for the structural interrogation of proteins in the sub-20 nm range? Here, we show that the combination of DNA nanotechnology and single-molecule biochemistry allows the first step towards the investigation of the structural organization of a protein via SR microscopy. Limiting factors and possible future directions for the full implementation of SR microscopy as a structural tool are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXPANSION MICROSCOPY; DNA ORIGAMI; FLUORESCENCE MICROSCOPY; RESOLUTION; NANOSCALE; MOLECULES; REVEALS; PROTEIN; ORGANIZATION; ANTIBODIES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann |
| Depositing User: | Petra Gürster |
| Date Deposited: | 09 Jul 2020 11:16 |
| Last Modified: | 09 Jul 2020 11:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13837 |
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