Kindt, Alida and Liebisch, Gerhard and Clavel, Thomas and Haller, Dirk and Hoermannsperger, Gabriele and Yoon, Hongsup and Kolmeder, Daniela and Sigruener, Alexander and Krautbauer, Sabrina and Seeliger, Claudine and Ganzha, Alexandra and Schweizer, Sabine and Morisset, Rosalie and Strowig, Till and Daniel, Hannelore and Helm, Dominic and Kuester, Bernhard and Krumsiek, Jan and Ecker, Josef (2018) The gut microbiota promotes hepatic fatty acid desaturation and elongation in mice. NATURE COMMUNICATIONS, 9: 3760. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Interactions between the gut microbial ecosystem and host lipid homeostasis are highly relevant to host physiology and metabolic diseases. We present a comprehensive multiomics view of the effect of intestinal microbial colonization on hepatic lipid metabolism, integrating transcriptomic, proteomic, phosphoproteomic, and lipidomic analyses of liver and plasma samples from germfree and specific pathogen-free mice. Microbes induce monounsaturated fatty acid generation by stearoyl-CoA desaturase 1 and polyunsaturated fatty acid elongation by fatty acid elongase 5, leading to significant alterations in glycerophospholipid acyl-chain profiles. A composite classification score calculated from the observed alterations in fatty acid profiles in germfree mice clearly differentiates antibiotic-treated mice from untreated controls with high sensitivity. Mechanistic investigations reveal that acetate originating from gut microbial degradation of dietary fiber serves as precursor for hepatic synthesis of C16 and C18 fatty acids and their related glycerophospholipid species that are also released into the circulation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; LIPID-METABOLISM; HOST ENERGY; QUANTIFICATION; FRACTIONATION; MECHANISMS; ENRICHMENT; ASSIGNMENT; SEQUENCES; ISOMERS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Dec 2019 14:48 |
| Last Modified: | 12 Dec 2019 14:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13859 |
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