Ruegamer, Tamara and Hoffmann, Rebecca and Rohrhofer, Anette and Audebert, Franz and Salzberger, Bernd and Korn, Klaus and Schuster, Philipp and Eichler, Jutta and Schmidt, Barbara (2018) Inhibition of HIV-1 infection by human pegivirus type 1-derived peptides is affected by human pegivirus type 1 genotype and HIV-1 coreceptor tropism. AIDS, 32 (14). pp. 1951-1957. ISSN 0269-9370, 1473-5571
Full text not available from this repository. (Request a copy)Abstract
Objective(s): Up to 40% of HIV-1 infected individuals are coinfected with human pegivirus type 1 (HPgV-1). The majority of studies, but not all, have reported a beneficial effect of HPgV-1 coinfection on HIV-1 disease progression. So far, the impact of different HPgV-1 genotypes on different HIV-1 subtypes remains unclear. Methods: Peptides derived from HPgV-1 envelope protein E2, and representing different viral genotypes, were synthesized using Fmoc/t-Bu-based solid phase peptide synthesis. The inhibitory effect of these peptides on the infection of reporter cell lines was tested using an HIV-1 subtype panel representing clades A (n = 2), AG (n = 2), B (n = 6), C (n = 2), D (n = 2), F (n = 2), G (n = 1), G/H (n = 1), and group O (n = 2). Results: HIV-1 infection was blocked more efficiently by peptides derived from HPgV-1 GT2 than GT1 (P = 0.05). The HIV-1 subtype did not affect the degree of inhibition by a peptide derived from HPgV-1 GT2. All CXCR4-/dual-tropic isolates (n = 12), but only half (four out of eight) CCR5-tropic viruses were inhibited by this peptide (P = 0.014). Conclusion: Our data indicate that the inhibitory effect of peptides derived from HPgV-1 E2 protein is dependent on the genotype, suggesting that coinfection with HPgV-1 GT1 is less likely to confer a beneficial effect on HIV-1 disease progression than GT2. The preferential suppression of more pathogenic CXCR4-tropic HIV-1 by peptides derived from HPgV-1 GT2 may explain the favorable effect in patients harboring these HIV-1 isolates. Consequently, HPgV-1 genotype and HIV-1 coreceptor tropism are likely determinants for the beneficial effect of HPgV-1 co-infection in HIV-1-infected individuals. Copyright (C) 2018 Wolters Kluwer Health, Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GB-VIRUS-C; C/HEPATITIS G VIRUS; HEPATITIS-G VIRUS; DISEASE PROGRESSION; NO INFLUENCE; CLADE-C; COINFECTION; ENTRY; ASSOCIATION; SURVIVAL; CCR5 coreceptors; CXCR4; GBV-C virus; hepatitis G virus; human immunodeficiency virus type 1; human pegivirus type 1; peptides |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Dec 2019 08:31 |
| Last Modified: | 13 Dec 2019 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13867 |
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