Uchida, Hitoshi and Matsumura, Shinji and Katano, Tayo and Watanabe, Masahiko and Schlossmann, Jens and Ito, Seiji (2018) Two isoforms of cyclic GMP-dependent kinase-I exhibit distinct expression patterns in the adult mouse dorsal root ganglion. MOLECULAR PAIN, 14: 1744806918. ISSN 1744-8069,
Full text not available from this repository. (Request a copy)Abstract
cGMP-dependent kinase-I (cGKI) is known to regulate spinal pain processing. This enzyme consists of two isoforms (cGKI alpha and cGKI beta) that show distinct substrate specificity and tissue distribution. It has long been believed that the alpha isoform is exclusively expressed in the adult dorsal root ganglion. The aim of the present study was to reexamine the expression of cGKI isoforms in the adult mouse dorsal root ganglion using isoform-specific cGKI antibodies whose specificities had been validated in the previous studies. Immunoblot and immunohistochemical analyses revealed the presence of both isoforms in the dorsal root ganglion. Moreover, cGKI alpha, was found to be mainly expressed within the cytoplasm of small- to medium-sized peptidergic and nonpeptidegic C-fibers, whereas cGKI beta was located within the nuclei of a wide range of dorsal root ganglion neurons. In addition, glutamine synthetase-positive satellite glial cells expressed both isoforms to varying degrees. Finally, using an experimental model for neuropathic pain produced by L5 spinal nerve transection, we found that cGKI alpha expression was downregulated in the injured, but not in the uninjured, dorsal root ganglion. In contrast, cGKI beta expression was upregulated in both the injured and uninjured dorsal root ganglions. Also, injury-induced cGKI beta upregulation was found to occur in small-to-medium-diameter dorsal root ganglion neurons. These data thus demonstrate the existence of two differently distributed cGKI isoforms in the dorsal root ganglion, and may provide insight into the cellular and molecular mechanisms of pain.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERIPHERAL-NERVE INJURY; REQUIRES NUCLEAR TRANSLOCATION; OXIDANT-INDUCED ACTIVATION; SATELLITE GLIAL-CELLS; PROTEIN-KINASE; NITRIC-OXIDE; NATRIURETIC PEPTIDE; NEUROPATHIC PAIN; SENSORY NEURONS; INFLAMMATORY HYPERALGESIA; Cyclic GMP-dependent kinase-I; dorsal root ganglion; satellite glial cell; peripheral nerve injury; gene expression |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Jan 2020 08:28 |
| Last Modified: | 10 Jan 2020 08:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14023 |
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