Just, Sarah and Mondot, Stanislas and Ecker, Josef and Wegner, Katrin and Rath, Eva and Gau, Laura and Streidl, Theresa and Hery-Arnaud, Genevieve and Schmidt, Sinah and Lesker, Till Robin and Bieth, Valentin and Dunker, Andreas and Strowig, Till and Hofmann, Thomas and Haller, Dirk and Liebisch, Gerhard and Gerard, Philippe and Rohn, Sascha and Lepage, Patricia and Clavel, Thomas (2018) The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism. MICROBIOME, 6: 134. ISSN 2049-2618,
Full text not available from this repository. (Request a copy)Abstract
Background: As the gut microbiota contributes to metabolic health, it is important to determine specific dietmicrobiota interactions that influence host metabolism. Bile acids and dietary fat source can alter phenotypes of diet-induced obesity, but the interplay with intestinal microorganisms is unclear. Here, we investigated metabolic consequences of diets enriched in primary bile acids with or without addition of lard or palm oil, and studied gut microbiota structure and functions in mice. Results: In combination with bile acids, dietary lard fed to male C57BL/6N mice for a period of 8 weeks enhanced fat mass accumulation in colonized, but not in germ-free mice when compared to palm oil. This was associated with impaired glucose tolerance, lower fasting insulin levels, lower counts of enteroendocrine cells, fatty liver, and elevated amounts of hepatic triglycerides, cholesteryl esters, and monounsaturated fatty acids. Lard-and bile acid-fed mice were characterized by shifts in dominant gut bacterial communities, including decreased relative abundances of Lachnospiraceae and increased occurrence of Desulfovibrionaceae and the species Clostridium lactatifermentans and Flintibacter butyricus. Metatranscriptomic analysis revealed shifts in microbial functions, including lipid and amino acid metabolism. Conclusions: Caution is required when interpreting data from diet-induced obesity models due to varying effects of dietary fat source. Detrimental metabolic consequences of a diet enriched with lard and primary bile acids were dependent on microbial colonization of the host and were linked to hepatic lipid rearrangements and to alterations of dominant bacterial communities in the cecum.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH-THROUGHPUT QUANTIFICATION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; INTESTINAL MICROBIOTA; INSULIN-RESISTANCE; LIPID EXTRACTION; INDUCED OBESITY; SP NOV.; MICE; CHOLESTEROL; LIVER; Metabolic diseases; Diet-induced obesity; Gut microbiota; Germ-free mice; Bile acids; Dietary fat; Lard; Lipidomics; 16S rRNA gene amplicon sequencing; Metatranscriptomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Feb 2020 14:33 |
| Last Modified: | 12 Feb 2020 14:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14067 |
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