Schulze, Markus and Sommer, Annika and Plotz, Sonja and Farrell, Michaela and Winner, Beate and Grosch, Janina and Winkler, Juergen and Riemenschneider, Markus J. (2018) Sporadic Parkinson's disease derived neuronal cells show disease-specific mRNA and small RNA signatures with abundant deregulation of piRNAs. ACTA NEUROPATHOLOGICA COMMUNICATIONS, 6: 58. ISSN 2051-5960,
Full text not available from this repository. (Request a copy)Abstract
Differentiated neurons established via iPSCs from patients that suffer from familial Parkinson's disease (PD) have allowed insights into the mechanisms of neurodegeneration. In the larger cohort of patients with sporadic PD, iPSC based information on disease specific cellular phenotypes is rare. We asked whether differences may be present on genomic and epigenomic levels and performed a comprehensive transcriptomic and epigenomic analysis of fibroblasts, iPSCs and differentiated neuronal cells of sporadic PD-patients and controls. We found that on mRNA level, although fibroblasts and iPSCs are largely indistinguishable, differentiated neuronal cells of sporadic PD patients show significant alterations enriched in pathways known to be involved in disease aetiology, like the CREB-pathway and the pathway regulating PGC1a. Moreover, miRNAs and piRNAs/piRNA-like molecules are largely differentially regulated in cells and post-mortem tissue samples between control-and PD-patients. The most striking differences can be found in piRNAs/piRNA-like molecules, with SINE-and LINE-derived piRNAs highly downregulated in a disease specific manner. We conclude that neuronal cells derived from sporadic PD-patients help to elucidate novel disease mechanisms and provide relevant insight into the epigenetic landscape of sporadic Parkinson's disease as particularly regulated by small RNAs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PLURIPOTENT STEM-CELLS; ALZHEIMERS-DISEASE; DOPAMINE NEURONS; NONCODING RNAS; COPY NUMBER; METHYLATION; MEMORY; DATABASE; BRAIN; EXPRESSION; Methylation; PD; Induced pluripotent stem cells; iPSC; SINE; LINE |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Neuropathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Feb 2020 11:29 |
| Last Modified: | 13 Feb 2020 11:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14243 |
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