Schneider, Christin and Gebhardt, Lisa and Arndt, Stephanie and Karrer, Sigrid and Zimmermann, Julia L. and Fischer, Michael J. M. and Bosserhoff, Anja-Katrin (2018) Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence. SCIENTIFIC REPORTS, 8: 10048. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Cold atmospheric plasma (CAP) is a promising approach in anti-cancer therapy, eliminating cancer cells with high selectivity. However, the molecular mechanisms of CAP action are poorly understood. In this study, we investigated CAP effects on calcium homeostasis in melanoma cells. We observed increased cytoplasmic calcium after CAP treatment, which also occurred in the absence of extracellular calcium, indicating the majority of the calcium increase originates from intracellular stores. Application of previously CAP-exposed extracellular solutions also induced cytoplasmic calcium elevations. A substantial fraction of this effect remained when the application was delayed for one hour, indicating the chemical stability of the activating agent(s). Addition of ryanodine and cyclosporin A indicate the involvement of the endoplasmatic reticulum and the mitochondria. Inhibition of the cytoplasmic calcium elevation by the intracellular chelator BAPTA blocked CAP-induced senescence. This finding helps to understand the molecular influence and the mode of action of CAP on tumor cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERMEABILITY TRANSITION PORE; NONTHERMAL PLASMA; MITOCHONDRIAL DYSFUNCTION; CYTOCHROME-C; CANCER; INDUCTION; APOPTOSIS; RELEASE; DEATH; INHIBITION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Feb 2020 13:34 |
| Last Modified: | 12 Feb 2020 13:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14263 |
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