Beutner, Andrea and Scherer, Beate and Matysik, Frank-Michael (2018) Dual detection for non-aqueous capillary electrophoresis combining contactless conductivity detection and mass spectrometry. TALANTA, 183. pp. 33-38. ISSN 0039-9140, 1873-3573
Full text not available from this repository. (Request a copy)Abstract
Coupling of two detectors is a powerful tool to enhance the overall analytical performance generating complementary information and overcoming the limitations of the single detectors. In this work, capacitively coupled contactless conductivity detection ((CD)-D-4) and electrospray ionization mass spectrometry (ESI-MS) were coupled in conjunction with non-aqueous capillary electrophoresis (NACE). Non-aqueous electrolytes are highly compatible with ESI due to their volatility. Moreover, they exhibit low background conductivity, which is essential for the detection with (CD)-D-4. A NACE-(CD)-D-4-MS method was developed using an acetonitrile buffer containing 2 M HAc and 4 mM NH4Ac as background electrolyte. The influence of the inner diameter of the separation capillary on the (CD)-D-4 was studied and taken into account. A capillary with 50 mu m inner diameter was found to be best suited. The complementarity of the two detectors was shown by determining a sample mixture containing choline, thiamine, nitrate, and chloride as well as bromide and acetylcholine as internal standards. The C4D was the detector of choice for the inorganic ions, which were not detectable with the MS whereas the MS had much lower limits of detections for the organic biomolecules. The method was applied on an extract of a food supplement containing the model analytes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTROCHEMICAL DETECTION; ELECTROSPRAY-IONIZATION; SEPARATION; MICROCHIP; CE; MS; SOLVENTS; ELECTROCHEMILUMINESCENCE; FLUORESCENCE; (CD)-D-4; Contactless conductivity detection; Electrospray ionization; Mass spectrometry; Dual detection; Non-aqueous capillary electrophoresis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Feb 2020 14:13 |
| Last Modified: | 17 Feb 2020 14:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14449 |
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