Jiang, Jiebing and Sun, Xueni and She, Xiaojian and Li, Jiajia and Li, Yan and Deng, Chunhui and Duan, Gengli (2018) Magnetic microspheres modified with Ti(IV) and Nb(V) for enrichment of phosphopeptides. MICROCHIMICA ACTA, 185 (6): UNSP 309. ISSN 0026-3672, 1436-5073
Full text not available from this repository. (Request a copy)Abstract
Magnetic microspheres (Fe3O4) were coated with polydopamine (PDA) and loaded with the metal ions Ti(IV) and Nb(V) to give a material of type Fe3O4@PDA-Ti/Nb. It is shown to be useful for affinity chromatography and for enrichment of phosphopeptides from both standard protein solutions and real samples. For comparison, such microspheres loaded with single metal ions only (Fe3O4@PDA-Ti and Fe3O4@PDA-Nb) and their physical mixtures were also investigated under identical conditions. The binary metal ion-loaded magnetic microspheres display better enrichment efficiency than the single metal ion-loaded microspheres and their physical mixture. Both multiphosphopeptides and monophosphopeptides can be extracted. The Fe3O4@PDA-Ti/Nb microspheres exhibit ultra-high sensitivity (the lowest detection amount being 2 fmol) and selectivity at a low mass ratio such as in case of beta-casein/BSA (1:1000).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ION AFFINITY-CHROMATOGRAPHY; HIGHLY SELECTIVE ENRICHMENT; METAL-ORGANIC FRAMEWORK; PHOSPHORYLATED PEPTIDES; PROTEIN-PHOSPHORYLATION; MASS-SPECTROMETRY; PHOSPHOPROTEOME RESEARCH; MALDI; GRAPHENE; NANOPARTICLES; Metal ions combination; Immobilized metal ion affinity chromatography; MALDI-TOF mass spectrometry; Polydopamine; Affinity probe |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Feb 2020 13:10 |
| Last Modified: | 17 Feb 2020 13:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14505 |
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