Chen, Junyi and Kalinowska, Kamila and Mueller, Benedikt and Mergner, Julia and Deutzmann, Rainer and Schwechheimer, Claus and Hammes, Ulrich Z. and Dresselhaus, Thomas (2018) DiSUMO-LIKE Interacts with RNA-Binding Proteins and Affects Cell-Cycle Progression during Maize Embryogenesis. CURRENT BIOLOGY, 28 (10). 1548-+. ISSN 0960-9822, 1879-0445
Full text not available from this repository. (Request a copy)Abstract
Embryogenesis in flowering plants is initiated by an asymmetric zygote division, generating two daughter cells that are the precursors of different cell lineages. Little is known about the molecular players regulating activation and progression of zygote development, establishment of asymmetry, and the plant-specific process of cell-plate formation. Here, we report the function of the ubiquitin-like modifier DiSUMO-LIKE (DSUL) for early embryo development in maize. Introducing a DSUL-RNAi construct by sperm cells affects cytokinesis generating non-separated zygotic daughter nuclei or multinucleate embryonic cells lacking cell plates. DSUL accumulates in the cytoplasm partly in granules, in the nucleus, as well as in the cell division zone. The enzymatic DSULyation cascade involves maturation and thesame enzymatic machinery for activation and conjugation as was previously shown for SUMO1. Identification of DSUL targets suggests predominant roles of DSULylation in regulation of cytoplasmic RNA metabolism as well as in cell-cycle progression and cell-plate formation. A comparison of DSUL and SUMO1 localization during the cell cycle and of their substrates indicates strong functional diversification between these two SUMO family modifiers.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ASYMMETRIC DIVISION; SUMO; SUMOYLATION; CONJUGATION; MODIFIER; TRANSFORMATION; ASSOCIATION; ARABIDOPSIS; REGULATOR; MECHANISM; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Feb 2020 14:03 |
| Last Modified: | 17 Feb 2020 14:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14568 |
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