Espinar-Marchena, Francisco and Rodriguez-Galan, Olga and Fernandez-Fernandez, Jose and Linnemann, Jan and de la Cruz, Jesus (2018) Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. NUCLEIC ACIDS RESEARCH, 46 (9). pp. 4715-4732. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
The contribution of most ribosomal proteins to ribosome synthesis has been quite well analysed in Saccharomyces cerevisiae. However, few yeast ribosomal proteins still await characterization. Herein, we show that L14, an essential 60S ribosomal protein, assembles in the nucleolus at an early stage into pre-60S particles. Depletion of L14 results in a deficit in 60S subunits and defective processing of 27SA(2) and 27SA(3) to 27SB pre-rRNAs. As a result, 27S pre-rRNAs are subjected to turnover and export of pre-60S particles is blocked. These phenotypes likely appear as the direct consequence of the reduced pre-60S particle association not only of L14 upon its depletion but also of a set of neighboring ribosomal proteins located at the solvent interface of 60S subunits and the adjacent region surrounding the polypeptide exit tunnel. These pre-60S intermediates also lack some essential trans-acting factors required for 27SB pre-rRNA processing but accumulate practically all factors required for processing of 27SA(3) pre-rRNA. We have also analysed the functional interaction between the eukaryote-specific carboxy-terminal extensions of the neighboring L14 and L16 proteins. Our results indicate that removal of the most distal parts of these extensions cause slight translation alterations in mature 60S subunits.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PUTATIVE RNA HELICASE; EUKARYOTIC RIBOSOME; NUCLEAR EXPORT; TRANSLATION INITIATION; CYTOPLASMIC MATURATION; EARLIEST PRECURSORS; REMODELING EVENTS; CRYSTAL-STRUCTURE; PROCESSING STEPS; BINDING DOMAIN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2020 13:25 |
| Last Modified: | 09 Mar 2020 13:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14570 |
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