Fernandes, Aleteia M. M. and Vilela, Polyana G. F. and Valera, Marcia C. and Bolay, Carola and Hiller, Karl Anton and Schweikl, Helmut and Schmalz, Gottfried (2018) Effect of bleaching agent extracts on murine macrophages. CLINICAL ORAL INVESTIGATIONS, 22 (4). pp. 1771-1781. ISSN 1432-6981, 1436-3771
Full text not available from this repository. (Request a copy)Abstract
Objectives The aim of this study was to evaluate the cytotoxicity and the influence of bleaching agents on immunologically cell surface antigens of murine macrophages in vitro. Materials and methods RAW 264.7 cells were exposed to bleaching gel extracts (40% hydrogen peroxide or 20% carbamide peroxide) and different H2O2 concentrations after 1 and 24-h exposure periods and 1-h exposure and 23-h recovery. Tests were performed with and without N-acetyl cysteine (NAC) and buthionine sulfoximine (BSO). Cell viability was determined by MTT assay. The expression of surface markers CD14, CD40, and CD54 with and without LPS stimulation was detected by flow cytometry, while the production of TNF-alpha was measured by ELISA. Statistical analysis was performed using the Mann-Whitney U test (alpha = 0.05). Results Extracts of bleaching agents were cytotoxic for cells after a 1-h exposure; cells could not recover after 24 h. This effect can be mitigated by the antioxidant NAC and increased by BSO, an inhibitor of glutathione (GSH) synthesis. LPS stimulated expression of all surface markers and TNF-alpha production. Exposure to bleaching agent extracts and H2O2 leads to a reduction of TNF-alpha, CD14, and CD40 expression, while the expression of CD54 was upregulated at non-cytotoxic concentrations. Whereas NAC reduced this effect, it was increased in the presence of BSO. Conclusions Extracts of bleaching agents were irreversibly cytotoxic to macrophages after a 1-h exposure. Only the expression of CD54 was upregulated. The reactions are mediated by the non-enzymatic antioxidant GSH.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYDROGEN-PEROXIDE DIFFUSION; SURFACE-ANTIGEN EXPRESSION; DENTAL RESIN MONOMERS; OXIDATIVE STRESS; DNA-DAMAGE; PULP CELLS; CARBAMIDE PEROXIDE; CYTOTOXICITY; RESPONSES; TOXICITY; Cytotoxicity; Immunology; Dental bleaching; Cytokines; Hydrogen peroxide |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Mar 2020 13:54 |
| Last Modified: | 19 Mar 2020 13:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14685 |
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