Dimartino, Dacia and Colantoni, Alessio and Ballarino, Monica and Martone, Julie and Mariani, Davide and Danner, Johannes and Bruckmann, Astrid and Meister, Gunter and Morlando, Mariangela and Bozzoni, Irene (2018) The Long Non-coding RNA lnc-31 Interacts with Rock1 mRNA and Mediates Its YB-1-Dependent Translation. CELL REPORTS, 23 (3). pp. 733-740. ISSN 2211-1247,
Full text not available from this repository. (Request a copy)Abstract
Cytoplasmic long non-coding RNAs have been shown to act at many different levels to control post-transcriptional gene expression, although their role in translational control is poorly understood. Here, we show that lnc-31, a non-coding RNA required for myoblast proliferation, promotes ROCK1 protein synthesis by stabilizing its translational activator, YB-1. We find that lnc-31 binds to the Rock1 mRNA as well as to the YB-1 protein and that translational activation requires physical interaction between the two RNA species. These results suggest a localized effect of YB-1 stabilization on the Rock1 mRNA. ROCK1 upregulation by lnc-31, in proliferative conditions, correlates well with the differentiation-repressing activity of ROCK1. We also show that, upon induction of differentiation, the downregulation of lnc-31, in conjunction with miR-152 targeting of Rock1, establishes a regulatory loop that reinforces ROCK1 repression and promotes myogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | YB-1 PROMOTES; MYOGENIC DIFFERENTIATION; MUSCLE DIFFERENTIATION; SKELETAL-MUSCLE; MYOBLAST FUSION; NEURAL ACTIVITY; EXPRESSION; TRANSCRIPTION; LOCALIZATION; ACTIVATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 07:29 |
| Last Modified: | 20 Mar 2020 07:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14720 |
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