Gust, Alexander and Jakob, Leonhard and Zeitler, Daniela M. and Bruckmann, Astrid and Kramm, Kevin and Willkomm, Sarah and Tinnefeld, Philip and Meister, Gunter and Grohmann, Dina (2018) Site-Specific Labelling of Native Mammalian Proteins for Single-Molecule FRET Measurements. CHEMBIOCHEM, 19 (8). pp. 780-783. ISSN 1439-4227, 1439-7633
Full text not available from this repository. (Request a copy)Abstract
Human cells are complex entities in which molecular recognition and selection are critical for cellular processes often driven by structural changes and dynamic interactions. Biomolecules appear in different chemical states, and modifications, such as phosphorylation, affect their function. Hence, using proteins in their chemically native state in biochemical and biophysical assays is essential. Single-molecule FRET measurements allow exploration of the structure, function and dynamics of biomolecules but cannot be fully exploited for the human proteome, as a method for the site-specific coupling of organic dyes into native, non-recombinant mammalian proteins is lacking. We address this issue showing the site-specific engineering of fluorescent dyes into human proteins on the basis of bioorthogonal reactions. We show the applicability of the method to study functional and post-translationally modified proteins on the single-molecule level, among them the hitherto inaccessible human Argonaute2.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-BINDING; ARGONAUTE; PHOSPHORYLATION; COMPLEXES; Argonaute; bioorganic chemistry; fluorescence; FRET; single-molecule studies; Staudinger ligation |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Dina Grohmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Mar 2020 13:09 |
| Last Modified: | 10 Mar 2020 13:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14722 |
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