Krickl, Sebastian and Touraud, Didier and Bauduin, Pierre and Zinn, Thomas and Kunz, Werner (2018) Enzyme activity of horseradish peroxidase in surfactant-free microemulsions. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 516. pp. 466-475. ISSN 0021-9797, 1095-7103
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In the present contribution, we investigated the influence of the structuring of surfactant-free microemulsions (SFME) (water/1-propanolilimonene and wateritert-butanolflimonene) on the enzyme activity of horseradish peroxidase (HRP). To this purpose, the oxidation of 2,2'-azino-bis(3-ethylbenzo thiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide was chosen as a model reaction. Enzymatic activities in SFMEs of varying compositions were investigated by UV-Vis spectroscopy and compared to the enzyme activity in pure buffer solution. Dynamic light, small-angle-X-ray scattering and conductivity measurements were performed in order to obtain structural information on the used SFMEs. Findings presented in this study revealed that the ability of short-chain alcohols to form mesostructures (aqueous aggregates in oil) has a crucial effect on the enzyme activity in SFME5. Mesoscale structuring with 1-propanol (NPA) was found to be more pronounced than for the more hydrophobic tertbutanol (TBA). It was concluded that the most pronounced mesoscale-structured SFMEs lead to the highest enzymatic activities. (C) 2018 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DYNAMIC LIGHT-SCATTERING; NANO-DROPLET FORMATION; DETERGENTLESS MICROEMULSIONS; MESOSCALE SOLUBILIZATION; PHASE-BEHAVIOR; SYSTEMS; MEDIA; WATER; MIXTURES; MORPHOLOGIES; Microemulsion; Enzyme; Activity; Surfactant-free; Surfactantless; Detergentless; Horseradish peroxidase; ABTS; HRP; Mesoscale structuring |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Feb 2020 13:22 |
| Last Modified: | 20 Feb 2020 13:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14723 |
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