Ljubimova, Julia Y. and Braubach, Oliver and Patil, Rameshwar and Chiechi, Antonella and Tang, Jie and Galstyan, Anna and Shatalova, Ekaterina S. and Kleinman, Michael T. and Black, Keith L. and Holler, Eggehard (2018) Coarse particulate matter (PM2.5-10) in Los Angeles Basin air induces expression of inflammation and cancer biomarkers in rat brains. SCIENTIFIC REPORTS, 8: 5708. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Air pollution is linked to brain inflammation, which accelerates tumorigenesis and neurodegeneration. The molecular mechanisms that connect air pollution with brain pathology are largely unknown but seem to depend on the chemical composition of airborne particulate matter (PM). We sourced ambient PM from Riverside, California, and selectively exposed rats to coarse (PM2.5-10: 2.5-10 mu m), fine (PM<2.5: < 2.5 mu m), or ultrafine particles (UFPM: < 0.15 mu m). We characterized each PM type via atomic emission spectroscopy and detected nickel, cobalt and zinc within them. We then exposed rats separately to each PM type for short (2 weeks), intermediate (1-3 months) and long durations (1 year). All three metals accumulated in rat brains during intermediate-length PM exposures. Via RNAseq analysis we then determined that intermediate-length PM2.5-10 exposures triggered the expression of the early growth response gene 2 (EGR2), genes encoding inflammatory cytokine pathways (IL13-R alpha 1 and IL-16) and the oncogene RAC1. Gene upregulation occurred only in brains of rats exposed to PM2.5-10 and correlated with cerebral nickel accumulation. We hypothesize that the expression of inflammation and oncogenesis-related genes is triggered by the combinatorial exposure to certain metals and toxins in Los Angeles Basin PM2.5-10.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AMBIENT ENDOTOXIN CONCENTRATIONS; ENRICHMENT SYSTEM VACES; SIMULTANEOUS IN-VIVO; OXIDATIVE STRESS; GLIOBLASTOMA-MULTIFORME; BIOLOGICAL CONSTITUENTS; RESTRICTIVE RECEPTOR; TRANSITION-METALS; VITRO EVALUATION; GENE-EXPRESSION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Alumni or Retired > Prof. Dr. Eggehard Holler |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Mar 2020 13:21 |
| Last Modified: | 10 Mar 2020 13:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14738 |
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