Cieplik, Fabian and Steinwachs, Viktoria-Sophia and Muehler, Denise and Hiller, Karl-Anton and Thurnheer, Thomas and Belibasakis, Georgios N. and Buchalla, Wolfgang and Maisch, Tim (2018) Phenalen-1-one-Mediated Antimicrobial Photodynamic Therapy: Antimicrobial Efficacy in a Periodontal Biofilm Model and Flow Cytometric Evaluation of Cytoplasmic Membrane Damage. FRONTIERS IN MICROBIOLOGY, 9: 688. ISSN 1664-302X,
Full text not available from this repository. (Request a copy)Abstract
In light of increasing resistance toward conventional antibiotics and antiseptics, antimicrobial photodynamic therapy (aPDT) may be a valuable alternative, especially for use in dentistry. In this regard, photosensitizers (PS) based on a phenalen-1-one structure seem to be especially favorable due to their high singlet oxygen quantum yield. However, the actual target structures of phenalen-1-one-mediated aPDT are still unclear. The aim of the present study was to investigate the antimicrobial efficacy of aPDT mediated by phenalen-1-one derivatives SAPYR and SAGUA for inactivation of a polymicrobial biofilm consisting of three putative periodontal pathogens in vitro and to get first insights in the mechanism of action of phenalen-1-one-mediated aPDT by assessing damage of cytoplasmic membranes. aPDT with SAPYR exhibited identical antimicrobial efficacy as compared to chlorhexidine (CHX) [4.4-6.1 log(10) reduction of colony forming units (CFUs) depending on bacterial species] while aPDT with SAGUA was less effective (2.0-2.8 log(10)). Flow cytometric analysis combined with propidium iodide (PI) staining revealed no damage of cytoplasmic membranes after aPDT with both phenalen-1-one derivatives, which was confirmed by spectroscopic measurements for release of nucleic acids after treatment. Spectrophotometric PS-uptake measurements showed no uptake of SAPYR by bacterial cells. Despite the inability to pinpoint the actual target of phenalen-1-one-mediated aPDT, this study shows the high antimicrobial potential of phenalen-1-on mediated aPDT (especially when using SAPYR) and represents a first step for getting insights in the mechanism and damage patterns of aPDT with this class of PS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SINGLET OXYGEN; BLUE-LIGHT; IN-VITRO; DEINOCOCCUS-RADIODURANS; STAPHYLOCOCCUS-AUREUS; SYSTEMIC AMOXICILLIN; STREPTOCOCCUS-MUTANS; BACTERIAL VIABILITY; ESCHERICHIA-COLI; INACTIVATION; biofilm; periodontal; photodynamic; phenalen-1-one; chlorhexidine; flow cytometry; propidium iodide; cytoplasmic membrane |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Mar 2020 13:24 |
| Last Modified: | 10 Mar 2020 13:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14741 |
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