Bartholomae, Maike and Baumann, Tobias and Nickling, Jessica H. and Peterhoff, David and Wagner, Ralf and Budisa, Nediljko and Kuipers, Oscar P. (2018) Expanding the Genetic Code of Lactococcus lactis and Escherichia coli to Incorporate Non-canonical Amino Acids for Production of Modified Lantibiotics. FRONTIERS IN MICROBIOLOGY, 9: 657. ISSN 1664-302X,
Full text not available from this repository. (Request a copy)Abstract
The incorporation of non-canonical amino acids (ncAAs) into ribosomally synthesized and post-translationally modified peptides, e.g., nisin from the Gram-positive bacterium Lactococcus lactis, bears great potential to expand the chemical space of various antimicrobials. The ncAA N-epsilon-Boc-L-lysine (BocK) was chosen for incorporation into nisin using the archaeal pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair to establish orthogonal translation in L. lactis for read-through of in-frame amber stop codons. In parallel, recombinant nisin production and orthogonal translation were combined in Escherichia coli cells. Both organisms synthesized bioactive nisin(BocK) variants. Screening of a nisin amber codon library revealed suitable sites for ncAA incorporation and two variants displayed high antimicrobial activity. Orthogonal translation in E. coli and L. lactis presents a promising tool to create new-to-nature nisin derivatives.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSFER-RNA SYNTHETASES; NISIN; PEPTIDES; ANTIBACTERIAL; MUTAGENESIS; BIOSYNTHESIS; CHEMISTRY; EXPANSION; MEMBRANE; SYSTEMS; stop codon suppression; pyrrolysyl-tRNA synthetase; nisin; orthogonal translation system; antimicrobial peptides; bacteriocin; non-canonical amino acids |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 07:43 |
| Last Modified: | 20 Mar 2020 07:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14742 |
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