Grund, Thomas and Neumann, Inga D. (2018) Neuropeptide S Induces Acute Anxiolysis by Phospholipase C-Dependent Signaling within the Medial Amygdala. NEUROPSYCHOPHARMACOLOGY, 43 (5). pp. 1156-1163. ISSN 0893-133X, 1740-634X
Full text not available from this repository. (Request a copy)Abstract
Neuropeptide S (NPS) is an important anxiolytic substance of the brain. However, the signaling pathways downstream of NPS receptor (NPSR) activation, underlying the behavioral effect of NPS, remain largely unknown. Here, we show that bilateral microinfusion of NPS (0.2 nmol/0.5 mu l) into the medial amygdala (MeA) of male adult Wistar rats reduced anxiety-related behavior on both the elevated plus-maze and the open field. Moreover, as shown in amygdala tissue micropunches intracerebroventricular infusion of NPS (1 nmol/5 mu l) (1) evoked phosphorylation and synthesis of CaMKII. in relation to reference protein beta-tubulin representing Ca2+ influx, and (2) induced phosphorylation of mitogen-activated protein kinase ERK1/2. The NPS-induced anxiolysis was prevented by local inhibition of phospholipase C signaling using U73122 (0.5 nmol/0.5 mu l) in the MeA, indicating the behavioral relevance of this pathway. Conversely, local pharmacological blockade of adenylyl cyclase signaling using 2', 5'-dideoxyadenosine (12.5 nmol/0.5 mu l) failed to inhibit the anxiolytic effect of NPS infused into the MeA. Hence, NPS promotes acute anxiolysis within the MeA dependent on NPSR-mediated phospholipase C signaling. Taken together, our study extends the knowledge about the intracellular signaling mechanisms underlying the potent anxiolytic profile of NPS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADENYLATE-CYCLASE; IN-VIVO; RECEPTOR; FEAR; NEURONS; ANXIETY; MEMORY; PROJECTIONS; EXTINCTION; AGONIST; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Mar 2020 12:46 |
| Last Modified: | 11 Mar 2020 12:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14865 |
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