Ramos-Gomes, Fernanda and Bode, Julia and Sukhanova, Alyona and Bozrova, Svetlana V. and Saccomano, Mara and Mitkovski, Miso and Krueger, Julia Eva and Wege, Anja K. and Stuehmer, Walter and Samokhvalov, Pavel S. and Baty, Daniel and Chames, Patrick and Nabiev, Igor and Alves, Frauke (2018) Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots. SCIENTIFIC REPORTS, 8: 4595. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5-10 mu m) paraffin and thick (50 mu m) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody-QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BREAST-CANCER CELLS; DOMAIN ANTIBODIES; FLUORESCENT NANOCRYSTALS; GROWTH; MICE; AUTOFLUORESCENCE; XENOGRAFTS; MODEL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 10:06 |
| Last Modified: | 20 Mar 2020 10:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14890 |
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