El-Najjar, Nahed and Hoesl, Julian and Holzmann, Thomas and Jantsch, Jonathan and , Andre (2018) UPLC-MS/MS method for therapeutic drug monitoring of 10 antibiotics used in intensive care units. DRUG TESTING AND ANALYSIS, 10 (3). pp. 584-591. ISSN 1942-7603, 1942-7611
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A large variation in the levels of different ss-lactams and other antibiotics used in critically ill patients has been documented. The aim of this study is to establish and validate a fast, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous analysis of ten antibiotics (Meropenem, Cefepime, Ceftazidime, Piperacillin, Benzylpenicillin, Ampicillin, Flucloxacillin, Linezolid, and Sulfamethoxazole/Trimethoprim) in human plasma according to European Medicines Agency (EMA) guidelines. Protein precipitation with ice-cold methanol containing 9 isotopically labeled internal standards was used for sample clean up. Antibiotics were detected, following a 4-minute gradient separation, in multiple reactions monitoring (MRM) using API 4000 instrument equipped with electrospray source operating in positive ion mode. The lower limit of quantification was 0.1 mg/L for Meropenem, Ceftazidime, Piperacillin, Ampicillin, Flucloxacillin, and Sulfamethoxazole; 0.05 mg/L for Cefepime, Benzylpenicillin, and Trimethoprim; and 0.02 mg/L for Linezolid. The method proved to be precise and accurate and applicable for therapeutic drug monitoring and other pharmacokinetic studies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BETA-LACTAM ANTIBIOTICS; TANDEM MASS-SPECTROMETRY; CRITICALLY-ILL PATIENTS; PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN PLASMA; SEPTIC PATIENTS; HUMAN SERUM; QUANTIFICATION; PHARMACOKINETICS; ANIDULAFUNGIN; antibiotics; critically ill patients; liquid chromatography; mass spectrometry; therapeutic drug monitoring |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Mar 2020 10:13 |
| Last Modified: | 19 Mar 2020 10:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/14963 |
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