Schwendner, Petra and Bohmeier, Maria and Rettberg, Petra and Beblo-Vranesevic, Kristina and Gaboyer, Frederic and Moissl-Eichinger, Christine and Perras, Alexandra K. and Vannier, Pauline and Marteinsson, Viggo T. and Garcia-Descalzo, Laura and Gomez, Felipe and Malki, Moustafa and Amils, Ricardo and Westall, Frances and Riedo, Andreas and Monaghan, Euan P. and Ehrenfreund, Pascale and Cabezas, Patricia and Walter, Nicolas and Cockell, Charles (2018) Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity. FRONTIERS IN MICROBIOLOGY, 9: 335. ISSN 1664-302X,
Full text not available from this repository. (Request a copy)Abstract
Growth in sodium chloride (NaCl) is known to induce stress in non-halophilic microorganisms leading to effects on the microbial metabolism and cell structure. Microorganisms have evolved a number of adaptations, both structural and metabolic, to counteract osmotic stress. These strategies are well-understood for organisms in NaCl-rich brines such as the accumulation of certain organic solutes (known as either compatible solutes or osmolytes). Less well studied are responses to ionic environments such as sulfate-rich brines which are prevalent on Earth but can also be found on Mars. In this paper, we investigated the global metabolic response of the anaerobic bacterium Yersinia intermedia MASE-LG-1 to osmotic salt stress induced by either magnesium sulfate (MgSO4) or NaCl at the same water activity (0.975). Using a non-targeted mass spectrometry approach, the intensity of hundreds of metabolites was measured. The compatible solutes L-asparagine and sucrose were found to be increased in both MgSO4 and NaCl compared to the control sample, suggesting a similar osmotic response to different ionic environments. We were able to demonstrate that Yersinia intermedia MASE-LG-1 accumulated a range of other compatible solutes. However, we also found the global metabolic responses, especially with regard to amino acid metabolism and carbohydrate metabolism, to be salt-specific, thus, suggesting ion-specific regulation of specific metabolic pathways.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPATIBLE SOLUTE ACCUMULATION; ESCHERICHIA-COLI; OSMOTIC-STRESS; HELICOBACTER-PYLORI; GLYCINE BETAINE; L-ASPARAGINE; SALT STRESS; ENTEROCOLITICA; TOLERANCE; BACTERIA; sodium chloride; magnesium sulfate; metabolome; compatible solutes; stress response |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Mar 2020 13:50 |
| Last Modified: | 16 Mar 2020 13:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15016 |
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