Knueppel, Robert and Christensen, Regitse H. and Gray, Fiona C. and Esser, Dominik and Strauss, Daniela and Medenbach, Jan and Siebers, Bettina and MacNeill, Stuart A. and LaRonde, Nicole and Ferreira-Cerca, Sebastien (2018) Insights into the evolutionary conserved regulation of Rio ATPase activity. NUCLEIC ACIDS RESEARCH, 46 (3). pp. 1441-1456. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
Eukaryotic ribosome biogenesis is a complex dynamic process which requires the action of numerous ribosome assembly factors. Among them, the eukaryotic Rio protein family members (Rio1, Rio2 and Rio3) belong to an ancient conserved atypical protein kinase/ATPase family required for the maturation of the small ribosomal subunit (SSU). Recent structure-function analyses suggested an ATPase-dependent role of the Rio proteins to regulate their dynamic association with the nascent pre-SSU. However, the evolutionary origin of this feature and the detailed molecular mechanism that allows controlled activation of the catalytic activity remained to be determined. In this work we provide functional evidence showing a conserved role of the archaeal Rio proteins for the synthesis of the SSU in archaea. Moreover, we unravel a conserved RNA-dependent regulation of the Rio ATPases, which in the case of Rio2 involves, at least, helix 30 of the SSU rRNA and the P-loop lysine within the shared RIO domain. Together, our study suggests a ribosomal RNA-mediated regulatory mechanism enabling the appropriate stimulation of Rio2 catalytic activity and subsequent release of Rio2 from the nascent pre-40S particle. Based on our findings we propose a unified release mechanism for the Rio proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRE-RIBOSOMAL-RNA; ARCHAEON HALOFERAX-VOLCANII; SACCHAROMYCES-CEREVISIAE; CYTOPLASMIC MATURATION; PRE-40S RIBOSOMES; BINDING PROTEINS; IN-VIVO; SUBUNIT; BIOGENESIS; YEAST; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Mar 2020 08:51 |
| Last Modified: | 18 Mar 2020 08:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15035 |
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