Sunderkoetter, Cord and Becker, Karsten and Kutzner, Heinz and Meyer, Thomas and Bloedorn-Schlicht, Norbert and Reischl, Udo and Nenoff, Pietro and Geissdoerfer, Walter and Graeser, Yvonne and Herrmann, Mathias and Kuehn, Joachim and Bogdan, Christian (2018) Molecular diagnosis of skin infections using paraffin-embedded tissue review and interdisciplinary consensus. JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT, 16 (2). pp. 139-147. ISSN 1610-0379, 1610-0387
Full text not available from this repository. (Request a copy)Abstract
Nucleic acid amplification techniques (NATs), such as PCR, are highly sensitive and specific methods that have become valuable supplements to culture and serology in the diagnosis of infectious disorders. However, especially when using formalin-fixed and paraffin-embedded tissue, these techniques are associated with both false-negative and false-positive results, a pitfall that is frequently misjudged. Representatives of the German Society of Hygiene and Microbiology (DGHM) and the German Society of Dermatology (DDG) therefore set out to develop a consensus in the form of a review article on the appropriate indications for NATs using paraffin-embedded tissue, its contraindications, and the key points to be considered in the pre-and post-analytical phase. Given that fresh, naive tissue is preferably to be used in the workup of a suspected infection, PCR analysis on paraffin sections represents an exception. The latter may be considered if an infection is suspected at a later point in time and fresh tissue has not been preserved or can no longer be obtained. Potential indications include confirmation of histologically suspected infections with Leishrnania spp., Bartonella spp., Rickettsia spp., or in case of ecthyma contagiosum. Infections with, for example, mycobacteria or RNA viruses, on the other hand, are not considered useful indications for NATs using paraffin sections. In order to avoid misinterpretation of test results, it is essential that laboratory reports on NATs using paraffin-embedded tissue contain information on the indication/diagnostic circumstances, the required and chosen pre-analytical steps, the limitations of the method, and on diagnostic alternatives.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GUIDELINES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Petra Gürster |
| Date Deposited: | 25 Jun 2020 10:01 |
| Last Modified: | 25 Jun 2020 10:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15072 |
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