Morsczeck, Christian and Hullmann, Markus and Reck, Anja and Reichert, Torsten E. (2018) The cell cycle regulator protein P16 and the cellular senescence of dental follicle cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 439 (1-2). pp. 45-52. ISSN 0300-8177, 1573-4919
Full text not available from this repository. (Request a copy)Abstract
Cellular senescence is a restricting factor for regenerative therapies with somatic stem cells. We showed previously that the onset of cellular senescence inhibits the osteogenic differentiation in stem cells of the dental follicle (DFCs), although the mechanism remains elusive. Two different pathways are involved in the induction of the cellular senescence, which are driven either by the cell cycle protein P21 or by the cell cycle protein P16. In this study, we investigated the expression of cell cycle proteins in DFCs after the induction of cellular senescence. The induction of cellular senescence was proved by an increased expression of beta-galactosidase and an increased population doubling time after a prolonged cell culture. Cellular senescence regulated the expression of cell cycle proteins. The expression of cell cycle protein P16 was up-regulated, which correlates with the induction of cellular senescence markers in DFCs. However, the expression of cyclin-dependent kinases (CDK)2 and 4 and the expression of the cell cycle protein P21 were successively decreased in DFCs. In conclusion, our data suggest that a P16-dependent pathway drives the induction of cellular senescence in DFCs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; IN-VITRO EXPANSION; QUANTITATIVE PCR; TELOMERE LENGTH; LIFE-SPAN; DIFFERENTIATION; EXPRESSION; GENE; VIVO; AGE; Dental follicle cells; Cellular senescence; Osteogenic differentiation; Cell cycle proteins |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 12:46 |
| Last Modified: | 20 Mar 2020 12:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15139 |
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