Yurova, Nadezhda S. and Danchuk, Alexandra and Mobarez, Sarah N. and Wongkaew, Nongnoot and Rusanova, Tatiana and Baeumner, Antje J. and Duerkop, Axel (2018) Functional electrospun nanofibers for multimodal sensitive detection of biogenic amines in food via a simple dipstick assay. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410 (3). pp. 1111-1121. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
Electrospun nanofibers (ENFs) are promising materials for rapid diagnostic tests like lateral flow assays and dipsticks because they offer an immense surface area while excluding minimal volume, a variety of functional surface groups, and can entrap functional additives within their interior. Here, we show that ENFs on sample pads are superior in comparison to standard polymer membranes for the optical detection of biogenic amines (BAs) in food using a dipstick format. Specifically, cellulose acetate (CA) fibers doped with 2 mg/mL of the chromogenic and fluorogenic amine-reactive chameleon dye Py-1 were electrospun into uniform anionic mats. Those extract cationic BAs from real samples and Py-1 transduces BA concentrations into a change of color, reflectance, and fluorescence. Dropping a BA sample onto the nanofiber mat converts the weakly fluorescent pyrylium dye Py-1 into a strongly red emitting pyridinium dye. For the first time, a simple UV lamp excites fluorescence and a digital camera acts as detector. The intensity ratio of the red to the blue channel of the digital image is dependent on the concentration of most relevant BAs indicating food spoilage from 10 to 250 mu M. This matches the permitted limits for BAs in foods and no false positive signals arise from secondary and tertiary amines. BA detection in seafood samples was also demonstrated successfully. The nanofiber mat dipsticks were up to sixfold more sensitive than those using a polymer membrane with the same dye embedded. Hence, nanofiber-based tests are not only superior to polymer-based dipstick assays, but will also improve the performance of established tests related to food safety, medical diagnostics, and environmental testing.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OPTICAL-DETECTION; SENSOR; FLUORESCENCE; Biogenic amines; Electrospun nanofibers; Rapid diagnostic; Food analysis; Fluorescence; Dipstick |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 06:35 |
| Last Modified: | 20 Mar 2020 06:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15246 |
Actions (login required)
 |
View Item |
