Bathke, Anja and Klemm, Denis and Gstoettner, Christoph and Bell, Christian and Kopf, Robert (2018) Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Perform Liquid Chromatography-Mass Spectrometry. LC GC EUROPE, 31 (1). pp. 10-21. ISSN 1471-6577,
Full text not available from this repository.Abstract
Characterization of protein modifications is an essential aspect of biopharmaceutical development. Traditionally, the characterization process of chromatographic peaks involves manual, larger-scale fractionation to obtain a sufficient amount of material for further analytical studies. This article presents a fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent quadrupole time-of-flight mass spectrometry (QTOF-MS) characterization. This innovative technique significantly accelerates MS peak characterization compared to traditional approaches and avoids the risk of unintended modifications of the variants as a result of the isolation process, for example, deamidation during storage of isoforms. This approach considerably reduces the required sample amount and can be used for the characterization of product-related impurities during early stage development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECOMBINANT MONOCLONAL-ANTIBODY; DEAMIDATION; SUCCINIMIDE; PROTEOMICS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 09:33 |
| Last Modified: | 20 Mar 2020 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15345 |
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