Aktas, Zeynep and Rao, Hongyu and Slauson, Sarah R. and Gabelt, B'Ann T. and Larsen, Inna V. and Sheridan, Rachael T. C. and Herrnberger, Leonie and Tamm, Ernst R. and Kaufman, Paul L. and Brandt, Curtis R. (2018) Proteasome Inhibition Increases the Efficiency of Lentiviral Vector-Mediated Transduction of Trabecular Meshwork. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 59 (1). pp. 298-310. ISSN 0146-0404, 1552-5783
Full text not available from this repository. (Request a copy)Abstract
PURPOSE: To determine if proteasome inhibition using MG132 increased the efficiency of FIV vector-mediated transduction in human trabecular meshwork (TM)-1 cells and monkey organ-cultured anterior segments (MOCAS). METHODS: TM-1 cells were pretreated for 1 hour with 0.5% dimethyl sulfoxide (DMSO; vehicle control) or 5 to 50 mu M MG132 and transduced with FIV.GFP (green fluorescent protein)- or FIV.mCherry-expressing vector at a multiplicity of transduction (MOT) of 20. At 24 hours, cells were fixed and stained with antibodies for GFP, and positive cells were counted, manually or by fluorescence-activated cell sorting (FACS). Cells transduced with FIV.GFP particles alone were used as controls. The effect of 20 mu M MG132 treatment on high- and low-dose (2 x 10(7) and 0.8 x 10(7) transducing units [TU], respectively) FIV.GFP transduction with or without MG132 was also evaluated in MOCAS using fluorescence microscopy. Vector genome equivalents in cells and tissues were quantified by quantitative (q)PCR on DNA. RESULTS: In the MG132 treatment groups, there was a significant dose-dependent increase in the percentage of transduced cells at all concentrations tested. Vector genome equivalents were also increased in TM-1 cells treated with MG132. Increased FIV.GFP expression in the TM was also observed in MOCAS treated with 20 mu M MG132 and the high dose of vector. Vector genome equivalents were also significantly increased in the MOCAS tissues. Increased transduction was not seen with the low dose of virus. CONCLUSIONS: Proteasome inhibition increased the transduction efficiency of FIV particles in TM-1 cells and MOCAS and may be a useful adjunct for delivery of therapeutic genes to the TM by lentiviral vectors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-KINASE INHIBITOR; DOMINANT-NEGATIVE RHOA; OUTFLOW FACILITY; GENE-TRANSFER; TRANSGENE EXPRESSION; INTRAOCULAR-PRESSURE; UVEOSCLERAL OUTFLOW; CULTURED HUMAN; OPHTHALMIC SOLUTION; ANTERIOR SEGMENTS; trabecular meshwork; FIV vector; MG132; gene therapy proteasomes |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie > Prof. Dr. Ernst Tamm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 12:04 |
| Last Modified: | 20 Mar 2020 12:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15412 |
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