Weghofer, Margit and Grote, Monika and Resch, Yvonne and Casset, Anne and Kneidinger, Michael and Kopec, Jolanta and Thomas, Wayne R. and Fernandez-Caldas, Enrique and Kabesch, Michael and Ferrara, Rosetta and Mari, Adriano and Purohit, Ashok and Pauli, Gabrielle and Horak, Friedrich and Keller, Walter and Valent, Peter and Valenta, Rudolf and Vrtala, Susanne (2013) Identification of Der p 23, a Peritrophin-like Protein, as a New Major Dermatophagoides pteronyssinus Allergen Associated with the Peritrophic Matrix of Mite Fecal Pellets. JOURNAL OF IMMUNOLOGY, 190 (7). pp. 3059-3067. ISSN 0022-1767,
Full text not available from this repository. (Request a copy)Abstract
The house dust mite (HDM) Dermatophagoides pteronyssinus is one of most important allergen sources and a major elicitor of allergic asthma. We screened a D. pteronyssinus expression cDNA library with IgE Abs from HDM allergic patients. A cDNA coding for a new major allergen was isolated, which showed sequence homology to peritrophins, which contain chitin-binding domains and are part of the peritrophic matrix lining the gut of arthropods. The mature Der p 23 allergen was expressed in Escherichia coli as an 8-kDa protein without its hydrophobic leader sequence and purified to homogeneity. It reacted with IgE Abs from 74% of D. pteronyssinus allergic patients (n = 347) at levels comparable to the two major HDM allergens, Der p 1 and Der p 2. Thus, Der p 23 represents a new major D. pteronyssinus allergen. Furthermore, rDer p 23 exhibited high allergenic activity as demonstrated by upregulation of CD203c expression on basophils from D. pteronyssinus allergic patients. Immunogold electron microscopy localized the allergen in the peritrophic matrix lining the midgut of D. pteronyssinus as well as on the surface of the fecal pellets. Thus, we identified a new major D. pteronyssinus allergen as peritrophin-like protein. The high allergenic activity of Der p 23 and its frequent recognition as respiratory allergen may be explained by the fact that it becomes airborne and respirable through its association with mite feces. Der p 23 may be an essential component for diagnosis and specific immunotherapy of HDM allergy. The Journal of Immunology, 2013, 190: 3059-3067.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONSERVED DOMAIN DATABASE; HOUSE-DUST; BRONCHIAL-ASTHMA; RECOMBINANT; IMMUNOTHERAPY; DER-P-1; CDNA; GUT; CHITINASES; EXPRESSION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kinder- und Jugendmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Apr 2020 07:41 |
| Last Modified: | 20 Apr 2020 07:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16935 |
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