Elbakry, Asmaa and Wurster, Eva-Christina and Zaky, Alaa and Liebl, Renate and Schindler, Edith and Bauer-Kreisel, Petra and Blunk, Torsten and Rachel, Reinhard and Goepferich, Achim and Breunig, Miriam (2012) Layer-by-Layer Coated Gold Nanoparticles: Size-Dependent Delivery of DNA into Cells. SMALL, 8 (24). pp. 3847-3856. ISSN 1613-6810, 1613-6829
Full text not available from this repository. (Request a copy)Abstract
Because nanoparticles are finding uses in myriad biomedical applications, including the delivery of nucleic acids, a detailed knowledge of their interaction with the biological system is of utmost importance. Here the size-dependent uptake of gold nanoparticles (AuNPs) (20, 30, 50 and 80 nm), coated with a layer-by-layer approach with nucleic acid and poly(ethylene imine) (PEI), into a variety of mammalian cell lines is studied. In contrast to other studies, the optimal particle diameter for cellular uptake is determined but also the number of therapeutic cargo molecules per cell. It is found that 20 nm AuNPs, with diameters of about 32 nm after the coating process and about 88 nm including the protein corona after incubation in cell culture medium, yield the highest number of nanoparticles and therapeutic DNA molecules per cell. Interestingly, PEI, which is known for its toxicity, can be applied at significantly higher concentrations than its IC50 value, most likely because it is tightly bound to the AuNP surface and/or covered by a protein corona. These results are important for the future design of nanomaterials for the delivery of nucleic acids in two ways. They demonstrate that changes in the nanoparticle size can lead to significant differences in the number of therapeutic molecules delivered per cell, and they reveal that the toxicity of polyelectrolytes can be modulated by an appropriate binding to the nanoparticle surface.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FUNCTIONAL CORE/SHELL NANOPARTICLES; RECEPTOR-MEDIATED ENDOCYTOSIS; PLASMA-PROTEIN ADSORPTION; SIRNA DELIVERY; CELLULAR UPTAKE; DRUG-DELIVERY; AGGREGATION; POLYPLEXES; PARTICLES; STABILITY; layer-by-layer; poly(ethylene imine); DNA; cellular uptake; cytotoxicity |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 615 Pharmacy |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Apr 2020 07:25 |
| Last Modified: | 30 Apr 2020 07:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17583 |
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