Juranic, Martina and Srilunchang, Kanok-orn and Krohn, Nadia Graciele and Leljak-Levanic, Dunja and Sprunck, Stefanie and Dresselhaus, Thomas (2012) Germline-Specific MATH-BTB Substrate Adaptor MAB1 Regulates Spindle Length and Nuclei Identity in Maize. PLANT CELL, 24 (12). pp. 4974-4991. ISSN 1040-4651,
Full text not available from this repository. (Request a copy)Abstract
Germline and early embryo development constitute ideal model systems to study the establishment of polarity, cell identity, and asymmetric cell divisions (ACDs) in plants. We describe here the function of the MATH-BTB domain protein MAB1 that is exclusively expressed in the germ lineages and the zygote of maize (Zea mays). mab1 (RNA interference [RNAi]) mutant plants display chromosome segregation defects and short spindles during meiosis that cause insufficient separation and migration of nuclei. After the meiosis-to-mitosis transition, two attached nuclei of similar identity are formed in mab1 (RNAi) mutants leading to an arrest of further germline development. Transient expression studies of MAB1 in tobacco (Nicotiana tabacum) Bright Yellow-2 cells revealed a cell cycle-dependent nuclear localization pattern but no direct colocalization with the spindle apparatus. MAB1 is able to form homodimers and interacts with the E3 ubiquitin ligase component Cullin 3a (CUL3a) in the cytoplasm, likely as a substrate-specific adapter protein. The microtubule-severing subunit p60 of katanin was identified as a candidate substrate for MAB1, suggesting that MAB1 resembles the animal key ACD regulator Maternal Effect Lethal 26 (MEL-26). In summary, our findings provide further evidence for the importance of posttranslational regulation for asymmetric divisions and germline progression in plants and identified an unstable key protein that seems to be involved in regulating the stability of a spindle apparatus regulator(s).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FEMALE GAMETOPHYTE DEVELOPMENT; ARABIDOPSIS-THALIANA L; UBIQUITIN-LIGASE; FLOWERING PLANTS; C-ELEGANS; MICROTUBULAR CYTOSKELETON; MITOTIC PROGRESSION; SEQUENCE ALIGNMENT; EMBRYO DEVELOPMENT; SCUTELLAR TISSUE; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 05:03 |
| Last Modified: | 04 May 2020 05:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17629 |
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