Bollheimer, L. Cornelius and Buettner, Roland and Pongratz, Georg and Brunner-Ploss, Rita and Hechtl, Christine and Banas, Miriam and Singler, Katrin and Hamer, Okka W. and Stroszczynski, Christian and Sieber, Cornel C. and Fellner, Claudia (2012) Sarcopenia in the aging high-fat fed rat: a pilot study for modeling sarcopenic obesity in rodents. BIOGERONTOLOGY, 13 (6). pp. 609-620. ISSN 1389-5729,
Full text not available from this repository. (Request a copy)Abstract
Obesity has been suggested as a risk factor for sarcopenia. However, the underlying pathogenic concept of sarcopenic obesity is mainly based on phenotypical data from clinical observation. The present pilot study describes a rodent animal model which opens up prospects to carry out translational research of sarcopenic obesity in an experimental setting. Starting with 2 months, male Wistar rats were fed with a diet containing either 25 en % (control diet, CD) versus 45 en % (high fat diet, HFD) of neutral fat. At the age of 20 and 23 months quadriceps muscles were examined in vivo by magnetic resonance techniques which revealed a positive correlation between muscular fat and body weight (r = 0.639) and a negative correlation between muscular fat content and muscle volume (r = -0.742). Expression and phosphorylation status of proteins within the PKB/Akt and AMPK-dependent signaling pathway were examined in muscles of the 24 month-old animals which significantly showed a 50 percent upregulation of Ser(473)P-PKB/Akt and a 90 % constitutive downregulation of S6K1 in the HFD rats. Notably, S6K1 is a key mediator for muscular protein biosynthesis with additional negative feedback on PKB/Akt. Furthermore, muscular expression of the mitochondrial key regulator PGC-1 alpha in the aged HFD rats was only 25 % of that concurrent controls (p = 0.029). These explorative findings in the aging high-fat fed rat might serve as a firm starting point for controlled longitudinal observations in a larger animal cohort of both sexes studying the natural history of sarcopenic obesity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERMUSCULAR ADIPOSE-TISSUE; MUSCLE PROTEIN-SYNTHESIS; SKELETAL-MUSCLE; INSULIN-RESISTANCE; ACTIVATION; MYOSTATIN; AGE; QUANTIFICATION; DYSREGULATION; PGC-1-ALPHA; Sarcopenic obesity; High-fat diet; Myosteatosis; Magnetic resonance imaging; PI3K-Akt-pathway; S6K1; PGC1 alpha |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin II Medicine > Lehrstuhl für Röntgendiagnostik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 05:27 |
| Last Modified: | 04 May 2020 05:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17668 |
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