Viale-Bouroncle, Sandra and Gosau, Martin and Kuepper, Kevin and Moehl, Christoph and Brockhoff, Gero and Reichert, Torsten E. and Schmalz, Gottfried and Ettl, Tobias and Morsczeck, Christian (2012) Rigid matrix supports osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED). DIFFERENTIATION, 84 (5). pp. 366-370. ISSN 0301-4681,
Full text not available from this repository. (Request a copy)Abstract
Stem cell fate can be induced by the grade of stiffness of the extracellular matrix, depending on the developed tissue or complex tissues. For example, a rigid extracellular matrix induces the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs), while a softer surface induces the osteogenic differentiation in dental follicle cells (DFCs). To determine whether differentiation of ectomesenchymal dental precursor cells is supported by similar grades of extracellular matrices (ECMs) stiffness, we examined the influence of the surface stiffness on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED). Cell proliferation of SHED was significantly decreased on cell culture surfaces with a muscle-like stiffness. A dexamethasone-based differentiation medium induced the osteogenic differentiation of SHED on substrates of varying mechanical stiffness. Here, the hardest surface improved the induction of osteogenic differentiation in comparison to that with the softest stiffness. In conclusion, our study showed that the osteogenic differentiation of ectomesenchymal dental precursor cells SHED and DFCs are not supported by similar grades of ECM stiffness. (C) 2012 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENTAL FOLLICLE CELLS; IN-VITRO; ODONTOBLASTS; INTERPLAY; DYNAMICS; Stem cells from human exfoliated; deciduous teeth; Stiffness; PDMS; Differentiation; Proliferation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 05:37 |
| Last Modified: | 04 May 2020 05:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17670 |
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