Grosse, Jirka and Wehland, Markus and Pietsch, Jessica and Schulz, Herbert and Saar, Katrin and Huebner, Norbert and Eilles, Christoph and Bauer, Johann and Abou-El-Ardat, Khalil and Baatout, Sarah and Ma, Xiao and Infanger, Manfred and Hemmersbach, Ruth and Grimm, Daniela (2012) Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. FASEB JOURNAL, 26 (12). pp. 5124-5140. ISSN 0892-6638,
Full text not available from this repository. (Request a copy)Abstract
This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-kappa B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative real-time PCR using the same samples. Compared with 1-g IL-6, IL-8, CD44, and OPN were significantly up-regulated in AD cells but not in MCTSs, while ERK1/2, CAV2, TLN1, and CTGF were significantly down-regulated in AD cells. Simultaneously, the expression of ERK2, IL-6, CAV2, TLN1, and CTGF was reduced in MCTSs. IL-6 protein expression and secretion mirrored its gene expression. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-kappa B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.-Grosse, J., Wehland, M., Pietsch, J., Schulz, H., Saar, K., Hubner, N., Eilles, C., Bauer, J., Abou-El-Ardat, K., Baatout, S., Ma, X., Infanger, M., Hemmersbach, R., Grimm, D. Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. FASEB J. 26, 5124-5140 (2012). www.fasebj.org
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; RANDOM POSITIONING MACHINE; PLASMINOGEN-ACTIVATOR INHIBITOR-1; ENDOTHELIAL GROWTH-FACTOR; GENE-EXPRESSION PATTERNS; SIMULATED MICROGRAVITY; CARCINOMA CELLS; IN-VIVO; EXTRACELLULAR-MATRIX; PROTEIN; gene expression; simulated microgravity; cytokines; neoangiogenesis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 05:40 |
| Last Modified: | 04 May 2020 05:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17683 |
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