Schreml, Stephan and Meier, Robert J. and Weiss, Katharina T. and Cattani, Julia and Flittner, Dagmar and Gehmert, Sebastian and Wolfbeis, Otto S. and Landthaler, Michael and Babilas, Philipp (2012) A sprayable luminescent pH sensor and its use for wound imaging in vivo. EXPERIMENTAL DERMATOLOGY, 21 (12). pp. 951-953. ISSN 0906-6705,
Full text not available from this repository. (Request a copy)Abstract
Non-invasive luminescence imaging is of great interest for studying biological parameters in wound healing, tumors and other biomedical fields. Recently, we developed the first method for 2D luminescence imaging of pH in vivo on humans, and a novel method for one-stop-shop visualization of oxygen and pH using the RGB read-out of digital cameras. Both methods make use of semitransparent sensor foils. Here, we describe a sprayable ratiometric luminescent pH sensor, which combines properties of both these methods. Additionally, a major advantage is that the sensor spray is applicable to very uneven tissue surfaces due to its consistency. A digital RGB image of the spray on tissue is taken. The signal of the pH indicator (fluorescein isothiocyanate) is stored in the green channel (G), while that of the reference dye [ruthenium(II)-tris-(4,7-diphenyl-1,10-phenanthroline)] is stored in the red channel (R). Images are processed by rationing luminescence intensities (G/R) to result in pseudocolor pH maps of tissues, e.g. wounds.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACELLULAR PH; GENE-EXPRESSION; SKIN; OXYGEN; REGENERATION; REPAIR; cutaneous; luminescence; ratiometric; RGB imaging; skin |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Dermatologie und Venerologie Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 08:52 |
| Last Modified: | 04 May 2020 08:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17696 |
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