Sprunck, Stefanie and Rademacher, Svenja and Vogler, Frank and Gheyselinck, Jacqueline and Grossniklaus, Ueli and Dresselhaus, Thomas (2012) Egg Cell-Secreted EC1 Triggers Sperm Cell Activation During Double Fertilization. SCIENCE, 338 (6110). pp. 1093-1097. ISSN 0036-8075,
Full text not available from this repository. (Request a copy)Abstract
Double fertilization is the defining characteristic of flowering plants. However, the molecular mechanisms regulating the fusion of one sperm with the egg and the second sperm with the central cell are largely unknown. We show that gamete interactions in Arabidopsis depend on small cysteine-rich EC1 (EGG CELL 1) proteins accumulating in storage vesicles of the egg cell. Upon sperm arrival, EC1-containing vesicles are exocytosed. The sperm endomembrane system responds to exogenously applied EC1 peptides by redistributing the potential gamete fusogen HAP2/GCS1 (HAPLESS 2/GENERATIVE CELL SPECIFIC 1) to the cell surface. Furthermore, fertilization studies with ec1 quintuple mutants show that successful male-female gamete interactions are necessary to prevent multiple-sperm cell delivery. Our findings provide evidence that mutual gamete activation, regulated exocytosis, and sperm plasma membrane modifications govern flowering plant gamete interactions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SIGNAL-TRANSDUCTION; ARABIDOPSIS; PLANTS; FUSION; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 06:53 |
| Last Modified: | 04 May 2020 06:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17762 |
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