Sen, Amab and Nagy-Zsver-Vadas, Zsanett and Krahn, Michael P. (2012) Drosophila PATJ supports adherens junction stability by modulating Myosin light chain activity. JOURNAL OF CELL BIOLOGY, 199 (4). pp. 685-698. ISSN 0021-9525,
Full text not available from this repository. (Request a copy)Abstract
The assembly and consolidation of the adherens junctions (AJs) are key events in the establishment of an intact epithelium. However, AJs are further modified to obtain flexibility for cell migration and morphogenetic movements. Intact AJs in turn are a prerequisite for the establishment and maintenance of apical-basal polarity in epithelial cells. In this study, we report that the conserved PDZ (PSD95, Discs large, ZO-1) domain-containing protein PATJ (Pals1-associated tight junction protein) was not per se crucial for the maintenance of apical-basal polarity in Drosophila melanogaster epithelial cells but rather regulated Myosin localization and phosphorylation. PATJ directly bound to the Myosin-binding subunit of Myosin phosphatase and decreased Myosin dephosphorylation, resulting in activated Myosin. Thereby, PATJ supports the stability of the Zonula Adherens. Notably, weakening of AJ in a PATJ mutant epithelium led first to a loss of Myosin from the AJ, subsequently to a disassembly of the AJ, and finally, to a loss of apical-basal polarity and disruption of the tissue.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPITHELIAL-CELL POLARITY; APICAL-BASAL POLARITY; TIGHT-JUNCTION; PLANAR POLARITY; DISCS LOST; PHOTORECEPTOR MORPHOGENESIS; ZONULA ADHERENS; AXIS ELONGATION; VINCULIN GENE; COMPLEX; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 07:57 |
| Last Modified: | 04 May 2020 07:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17807 |
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