Pollinger, Klaus and Hennig, Robert and Breunig, Miriam and Tessmar, Joerg and Ohlmann, Andreas and Tamm, Ernst R. and Witzgall, Ralph and Goepferich, Achim (2012) Kidney Podocytes as Specific Targets for cyclo(RGDfC)-Modified Nanoparticles. SMALL, 8 (21). pp. 3368-3375. ISSN 1613-6810,
Full text not available from this repository. (Request a copy)Abstract
Renal nanoparticle passage opens the door for targeting new cells like podocytes, which constitute the exterior part of the renal filter. When cyclo(RGDfC)-modified Qdots are tested on isolated primary podocytes for selective binding to the alpha v beta 3 integrin receptor a highly cell- and receptor-specific binding can be observed. In displacement experiments with free cyclo(RGDfC) IC50 values of 150 nM for alpha v beta 3 integrin over-expressing U87-MG cells and 60 nM for podocytes are measured. Confocal microscopy shows a cellular Qdot uptake into vesicle-like structures. Our ex vivo study gives clear evidence that, after renal filtration, nanoparticles can be targeted to podocyte integrin receptors in the future. This could be a highly promising approach for future therapy and diagnostics of podocyte-associated diseases.
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