Marko, Lorant and Paragh, Gyoergy and Ugocsai, Peter and Boettcher, Alfred and Vogt, Thomas and Schling, Petra and Balogh, Attila and Tarabin, Victoria and Orso, Evelyn and Wikonkal, Norbert and Mandl, Jozsef and Remenyik, Eva and Schmitz, Gerd (2012) Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 116. pp. 79-88. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
Many ATP binding cassette (ABC) transporters are important regulators of lipid homeostasis and have been implicated in keratinocyte lipid transport. Ultraviolet (UV) light exposure is a known epidermal stressor, which amongst other effects causes lipid alterations and defective lamellar body biogenesis. To elucidate the background of these lipid changes we studied the effect of UVB light on ABC transporter expression. The effect of UVB treatment on the levels of 47 known human ABC transporter mRNAs was analyzed in normal human epidermal keratinocytes. Immunoblots and promoter assays were carried out for ABCA1 and ABCG1. The mRNA levels of cholesterol transport regulators ABCA1 and ABCG1 were markedly downregulated by UVB, parallel to the lamellar ichthyosis related glucosylceramide transporter ABCA12 and the suspected sphingosine-1-phosphate and cholesterol sulfate transporter ABCC1. The long but not the short alternative splice variant of the ABCF2 was found to be markedly upregulated rapidly after UVB irradiation. Immunoblot confirmed ABCA1 and ABCG1 protein downregulation, and luciferase assays showed suppression of their promoters by UVB. These proteins mostly transport lipids, which account for the integrity of the epidermal barrier; therefore our findings on the UVB regulation of ABC transporters may explain the appearance of barrier dysfunction after UVB exposure. (C) 2012 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CULTURED HUMAN KERATINOCYTES; UVB-INDUCED ALTERATIONS; STRATUM-CORNEUM LIPIDS; PERMEABILITY BARRIER; ABC TRANSPORTERS; SKIN BARRIER; HARLEQUIN ICHTHYOSIS; LAMELLAR GRANULES; IRRADIATION; GENE; Keratinocyte; Lipid transport; ABC transporter; UVB; Epidermal barrier |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 08:40 |
| Last Modified: | 04 May 2020 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17820 |
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