Schmid, A. and Kopp, A. and Hanses, F. and Bala, M. and Mueller, M. and Schaeffler, Andreas (2012) The Novel Adipokine C1q/TNF-related Protein-3 is Expressed in Human Adipocytes and Regulated by Metabolic and Infection-related Parameters. EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES, 120 (10). pp. 611-617. ISSN 0947-7349,
Full text not available from this repository. (Request a copy)Abstract
Background: The growing family of C1Q/TNF-related proteins is characterized by structural homologies to the anti-inflammatory adipokine adiponectin. CTRP-3 was recently reported to function as an anti-inflammatory LPS-antagonist in vitro. Material and methods: Human subcutaneous and visceral adipocytes and murine 3T3-L1 adipocytes were used for analysis of CTRP-3 expression and function. Western blot analysis of CTRP-3, siRNA mediated knockdown of CTRP-3, Oil red O staining, assessment of basal and epinephrine-induced lipolysis, ELISA-based measurements of supernatant chemokines, recombinant CTRP-3 protein expression, and Staphylococcus aureus (S. aureus) infection assays were used. Results: CTRP-3 is expressed in subcutaneous and visceral adipocytes. CTRP-3 is positively regulated by insulin, whereas chronic LPS-exposure inhibits terminal adipocyte differentiation and CTRP-3 expression. Intracellular infection of adipocytes by S. aureus also decreases CTRP-3 expression. As demonstrated by siRNA-mediated cellular knockdown of CTRP-3 in adipocytes, CTRP-3 regulates resistin secretion and lipolysis. Conclusion: CTRP-3 is expressed in human adipocytes and plays an important role in adipocyte physiology such as lipolysis and adipokine secretion. Both, metabolic factors and infection/inflammation-related factors regulate CTRP-3 expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REPEAT-CONTAINING SEQUENCE; ADIPOSE CELL LINE; COLLAGENOUS REPEAT; TRANSCRIPTIONAL REGULATION; CHROMOSOMAL LOCALIZATION; PROMOTES PROLIFERATION; GENOMIC ORGANIZATION; 26-KDA PROTEIN; PPAR-GAMMA; TISSUE; adipose tissue; CTRP-3; inflammation; lipolysis; adipokine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 09:51 |
| Last Modified: | 04 May 2020 09:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17836 |
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