Herrnberger, Leonie and Seitz, Roswitha and Kuespert, Sabrina and Boesl, Michael R. and Fuchshofer, Rudolf and Tamm, Ernst R. (2012) Lack of endothelial diaphragms in fenestrae and caveolae of mutant Plvap-deficient mice. HISTOCHEMISTRY AND CELL BIOLOGY, 138 (5). pp. 709-724. ISSN 0948-6143,
Full text not available from this repository. (Request a copy)Abstract
Plasmalemmal vesicle-associated protein (PLVAP, PV-1) is specifically expressed in endothelial cells in which it localizes to diaphragms of fenestrae, caveolae, and transendothelial channels. To learn about its function, we generated mutant mice that lack PLVAP. In a C57BL/6N genetic background, homozygous Plvap-deficient embryos die before birth and suffer from subcutaneous edema, hemorrhages, and defects in the vascular wall of subcutaneous capillaries. In addition, hearts of Plvap (-/-) embryos show ventricular septal defects and thinner ventricular walls. In wild-type embryos, PLVAP and caveolae with a stomatal diaphragm are present in endothelial cells of subcutaneous capillaries and endocardium, while a diaphragm is missing in caveolae of Plvap (-/-) littermates. Plvap (-/-) mice in a mixed C57BL/6N/FVB-N genetic background are born and survive at the most for 4 weeks. Capillaries of exocrine and endocrine pancreas and of kidney peritubular interstitium were investigated in more detail as examples of fenestrated capillaries. In these vascular beds, Plvap (-/-) mice show a complete absence of diaphragms in fenestrae, caveolae, and transendothelial channels, findings which are associated with a substantial decrease in the number of endothelial fenestrae. The changes in the capillary phenotype correlate with a considerable retardation of postnatal growth and anemia. Plvap (-/-) mice provide an animal model to clarify the specific functional role of endothelial fenestrae and their contribution to passage of water and solutes in different organs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECEPTOR TYROSINE KINASE; CAPILLARY ENDOTHELIUM; DIFFERENTIATED MICRODOMAINS; LUMINAL SURFACE; PHENOTYPIC HETEROGENEITY; ANIONIC SITES; KNOCKOUT MICE; DEFECTS; MOUSE; PV-1; Vascular endothelium; Capillaries; Kidney; Pancreas; Cardiac defects |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie > Prof. Dr. Ernst Tamm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2020 12:44 |
| Last Modified: | 04 May 2020 12:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17905 |
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