Nyuyki, Kewir D. and Maloumby, Rodrigue and Reber, Stefan O. and Neumann, Inga D. (2012) Comparison of corticosterone responses to acute stressors: Chronic jugular vein versus trunk blood samples in mice. STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, 15 (6). pp. 618-626. ISSN 1025-3890,
Full text not available from this repository. (Request a copy)Abstract
A commonly used method for obtaining blood samples from mice is decapitation. However, there is an obvious need for repeated blood sampling in mice under stress-free conditions. Here, we describe a simple technique to repeatedly collect blood samples from conscious, freely moving mice through a chronically implanted jugular vein catheter. Furthermore, we compare plasma corticosterone (CORT) concentrations in samples obtained through the catheter 1 day after surgery with samples taken from trunk blood obtained under basal or acute stress conditions. CORT concentrations in repeated 100-mu l venous blood samples were found to be similar to trunk blood samples both under basal conditions and after stressor exposure collected at identical time points (at 5, 15, and 60 min). Using both techniques, we demonstrate a progressive increase in CORT levels until 15 min after termination of stressor exposure and a decrease towards baseline values 60 min later. Anxiety-related behavior, as assessed on the elevated plus maze 3-4 days after surgery, did not differ between catheterized and non-catheterized mice. Our results provide evidence for application of jugular vein catheterization as a technique for repeated blood sampling in conscious laboratory mice. Use of this technique will greatly reduce the number of animals required for experiments involving endocrine endpoints.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PITUITARY-ADRENAL AXIS; ANXIETY-RELATED BEHAVIOR; VASOPRESSIN RELEASE; NEUROENDOCRINE RESPONSES; GLUCOSE-HOMEOSTASIS; RATS; OXYTOCIN; BRAIN; MICRODIALYSIS; MOUSE; Corticosterone; jugular vein catheterization; plus maze; stress; trunk blood; venous blood |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 May 2020 05:23 |
| Last Modified: | 05 May 2020 05:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17911 |
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