Scholbach, Johanna and Schulz, Anett and Westphal, Florian and Egger, Dietmar and Wege, Anja Kathrin and Patties, Ina and Koeberle, Margarethe and Sack, Ulrich and Lange, Franziska (2012) Comparison of Hematopoietic Stem Cells Derived from Fresh and Cryopreserved Whole Cord Blood in the Generation of Humanized Mice. PLOS ONE, 7 (10): e46772. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
To study the function and maturation of the human hematopoietic and immune system without endangering individuals, translational human-like animal models are needed. We compare the efficiency of CD34(+) stem cells isolated from cryopreserved cord blood from a blood bank (CCB) and fresh cord blood (FCB) in generating highly engrafted humanized mice in NOD-SCID IL2R gamma(null) (NSG) rodents. Interestingly, the isolation of CD34(+) cells from CCB results in a lower yield and purity compared to FCB. The purity of CD34(+) isolation from CCB decreases with an increasing number of mononuclear cells that is not evident in FCB. Despite the lower yield and purity of CD34(+) stem cell isolation from CCB compared to FCB, the overall reconstitution with human immune cells (CD45) and the differentiation of its subpopulations e.g., B cells, T cells or monocytes is comparable between both sources. In addition, independent of the cord blood origin, human B cells are able to produce high amounts of human IgM antibodies and human T cells are able to proliferate after stimulation with anti-CD3 antibodies. Nevertheless, T cells generated from FCB showed increased response to restimulation with anti-CD3. Our study reveals that the application of CCB samples for the engraftment of humanized mice does not result in less engraftment or a loss of differentiation and function of its subpopulations. Therefore, CCB is a reasonable alternative to FCB and allows the selection of specific genotypes (or any other criteria), which allows scientists to be independent from the daily changing birth rate.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMBINED IMMUNODEFICIENT MICE; CLASS-II GENES; CD34(+) CELLS; GAMMA-CHAIN; MOUSE MODEL; SUSCEPTIBILITY; ENGRAFTMENT; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 May 2020 06:05 |
| Last Modified: | 05 May 2020 06:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17950 |
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