Macabeo, Allan Patrick G. and Tudla, Florie A. and Krohn, Karsten and Franzblau, Scott G. (2012) Antitubercular activity of the semi-polar extractives of Uvaria rufa. ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 5 (10). pp. 777-780. ISSN 1995-7645,
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Objective: To investigate the inhibitory activity of the chloroform extract, petroleum ether and chloroform sub-extracts, lead-acetate treated chloroform extract, fractions and secondary metabolites of Uvaria rufa. (U. rufa.) against Mycobacterium tuberculosis (M. tuberculosis) H(37)Rv. Methods: The antituberculosis susceptibility assay was carried out using the colorimetric Microplate Alamar blue assay (MABA). In addition, the cytotoxicity of the most active fraction was evaluated using the VERO cell toxicity assay. Results: The in vitro inhibitory activity against M. tuberculosis H(37)Rv increased as purification progressed to fractionation (MIC up to 23 mu g/mL.). The chloroform extract and its sub-extracts showed moderate toxicity while the most active fraction from chloroform sub-extract exhibited no cytotoxicity against VERO cells. Meanwhile. the lead acetate-treated crude chloroform extract and its fractions showed complete inhibitions (100%) with MIC values up to 8 mu g/mL. Phytochemical screening of the most active fraction showed, in general, the presence of terpenoids, steroids and phenolic compounds. Evaluation of the antimycobacterial activity of known secondary metabolites isolated showed no promising inhibitory activity against the test organism. Conclusions: The present results demonstrate the potential of U. rufa as a phytomedicinal source of compounds that may exhibit promising antituberculosis activity. In addition, elimination of polar pigments revealed enhanced inhibition against M. tuberculosis H(37)Rv. While several compounds known for this plant did not show antimycobactetial activity, the obtained results are considered sufficient reason for further study to isolate the metabolites from U. rufa responsible for the antitubercular activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MYCOBACTERIUM-TUBERCULOSIS; IN-VITRO; INHIBITORS; ALKALOIDS; Uvaria rufa; Antitubercular; Mycobacterium tuberculosis H(37)Rv |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Anorganische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 May 2020 08:20 |
| Last Modified: | 05 May 2020 08:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17975 |
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